The Traditional microorganism primary identification method is through the principle that different kinds of bacteria use different kinds of sugar. There are several advantages to combine traditional microorganism identification methods with EWOD system. DMF(digital microfluidic) can simplify the loading steps to make it easier to operate. Utilizing EWOD system can reduce the volume of samples and reagents to about μL amount, so we expect to get results faster. Additionally, compared with traditional methods we can design to identify two different strains of bacteria at once.